3-(2-(8-(1H-benzimidazol-2-yl)quinolin-2-yl)ethenyl)-9-benzyl-9H-carbazole (probe L) was synthesized through multi-steps reaction, starting from anthranilic acid and carbazole. The structure was confirmed by 1HNMR, 13C NMR and MS. The research of fluorescence spectroscopy indicated that the probe L exhibited high selectivity to Zn2+, strong anti-interference capability, rapid response within 50 s and insensitivity at pH 5-9. A good linear work curve with R2=0.9999 had been achieved in the linearity range from 0 to 1.6×10-5 mol/L, and the limit of detection was 6.69×10-8 mol/L. A 1:1 binding ratio of Zn2+/L for the complex had been obtained by Job’s plot, fluorescence titration experiment and ESI-MS analysis method.