文章摘要
毕玉水,王凤泽.磁性纳米光敏剂对人癌细胞体外杀伤效应[J].精细化工,2018,35(6):0
磁性纳米光敏剂对人癌细胞体外杀伤效应
Killing Effect of Magnetic Nano Photosensitizer on Human Cancer Cells in Vitro
投稿时间:2017-04-18  修订日期:2017-08-03
DOI:
中文关键词: 纳米复合TiO2光敏剂  磁性  光动力  活性
英文关键词: nanocomposite TiO2 photosensitizer  magnetic property  photodynamic therapy  activity
基金项目:山东省自然科学基金(ZR2015EL010);国家级大学生创新创业训练计划(201510439030);山东省中医药管理局科技发展计划(2015-252);山东省安全生产科技发展计划(2016-89);泰安市科技发展计划(2015GX2045,2016GX1043)
作者单位E-mail
毕玉水 泰山医学院 yshbi@sina.com 
王凤泽 泰山医学院 生命科学学院  
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中文摘要:
      以硫酸亚铁、三氯化铁、正硅酸乙酯和四氯化钛为原料,通过沉淀、Stöber和溶胶凝胶法,制备得到一种复合结构的磁性纳米光敏剂TiO2/Fe3O4-SiO2,通过XRD、TEM、UV-Vis DRS和磁性测试等对其结构、性质进行了表征和分析,利用四甲基偶氮唑蓝比色法(MTT法)考察了该纳米复合光敏剂对A549人肺癌细胞的体外杀伤效应和不同TiO2负载量的TiO2/Fe3O4-SiO2对A549人肺癌细胞增殖的抑制率的影响。表征分析结果表明,该纳米复合光敏剂具有核壳结构和磁性,具有可见光吸收能力,能充分响应波长在400 nm以上的可见光。体外杀伤效应评价结果表明,当光照时间为150 min时,该纳米复合光敏剂和单纯的TiO2对A549人肺癌细胞增殖的抑制率分别为摩尔分数51%和36%,该纳米复合光敏剂具有增强的光动力活性。TiO2的适宜负载量在质量分数30%。
英文摘要:
      A composite structure of magnetic nano photosensitizer TiO2/Fe3O4-SiO2 has been prepared by precipitation, Stöber and sol-gel methods, using ferrous sulfate, ferric chloride, tetraethyl orthosilicate and titanium tetrachloride as raw materials. Structure and property of the nano composite photosensitizer were characterized and analyzed by means of XRD, TEM, UV-Vis DRS and magnetic test. Methyl thiazolyl tetrazolium colorimetric method (MTT method) was used to investigate the killing effect of nano composite photosensitizer on A549 human lung cancer cells in vitro, and effects of different TiO2 loadings of TiO2/Fe3O4-SiO2 on the inhibition ratios of A549 human lung cancer cells were also studied. The characterization results showed that the nano composite photosensitizer had core shell structure and magnetism, had visible light absorption capability, and could respond to visible light at wavelengths above 400 nm. In vitro cytotoxicity assay results showed that when the illumination time was 150 min, mole fractions of inhibiting proliferation of A549 human lung cancer cells for the nano composite photosensitizer and pure TiO2 were 51% and 36% respectively, the nano composite photosensitizer with enhanced photodynamic activity. The optimum load for TiO2 was 30% in mass fraction.
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