文章摘要
王杉杉,罗学刚,苏峰丙,田鹤.牦牛皮胶原蛋白的提取及其性能分析[J].精细化工,2018,35(5):
牦牛皮胶原蛋白的提取及其性能分析
Extraction and Characterization of Collagen from the Skin of Yak
投稿时间:2017-07-16  修订日期:2017-10-25
DOI:
中文关键词: 牦牛皮  胶原蛋白  热性质  超微结构  成纤维能力  D-周期
英文关键词: yak skin  collagen  thermal properties  ultrastructure  fibril forming ability  D-periodicity
基金项目:国家农业科技成果转化资金项目:(2012GB2G200469);西南科技大学研究生创新基金:(17ycx087)
作者单位E-mail
王杉杉 西南科技大学 674190725@qq.com 
罗学刚 西南科技大学 lxg@swust.edu.cn 
苏峰丙 西南科技大学  
田鹤 西南科技大学  
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中文摘要:
      4.以牦牛皮为原料,提取了酸溶性胶原蛋白(ASC)和酶溶性胶原蛋白(PSC),并对胶原蛋白分子的结构和性能进行了分析。结果表明:ASC和PSC的提取率分别为(54±0.18)%和(78±0.42)%;通过紫外吸收光谱(UV)分析、红外吸收光谱分析(FTIR)及电泳分析(SDS-PAGE),表明ASC和PSC具有完整的三股螺旋结构,符合Ⅰ型胶原蛋白的结构特征;氨基酸分析发现ASC和PSC含有丰富的亚氨酸,其含量分别为264.1残基/1000残基和276.6残基/1000残基;热稳定性分析表明ASC和PSC热变性温度为41.5 ℃、47.5 ℃,热收缩温度为62.5 ℃、70 ℃,证明PSC的热稳定性高于ASC;用扫描电子显微镜(SEM)观察ASC和PSC形貌,显示其表面为松散,不规则的纤维网形态;自组装实验说明两者具有一定的成纤维能力,自组装产物的D-周期分别为(68.2±5)nm、(69.3±3)nm,且PSC比ASC的组装速度快。
英文摘要:
      Yak skin was utilized to prepare the acid-soluble collagen (ASC) and pepsin-soluble collagen (PSC), whose structure and property had been analyzed. The results showed that the extraction rates of ASC and PSC were (54±0.18)% and (78±0.42)%, respectively. The results of ultraviolet absorption spectrum analysis, infrared absorption spectrum analysis and electrophoresis analysis showed that both ASC and PSC had a complete three strand helical structure, which conformed to the structural characteristics of I type collagen. Amino acid analysis indicated that ASC and PSC were rich in imidates at 264.1 residues/1000 residues and 276.6 residues/1000 residues. The thermal stability of ASC and PSC was 41.5 ℃, 47.5 ℃, and the heat shrinkage temperature was 62.5 ℃, 70 ℃. The thermal stability of PSC was higher than that of ASC. ASC and PSC were observed by scanning electron microscopy (SEM) to determin the loose and irregular morphology. Self-assembly experiment illustrated that both ASC and PSC performed the fiber forming ability. Self-assembly experiments showed that PSC and ASC have certain fiber-forming ability, and the D-periodicity of self-assembled products was (68.2±5) nm nm and (69.3±3) nm, respectively, and PSC was assembly faster than ASC.
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