Mitochondria are a major source of cellular reactive sulfur species (RSS). The RSS play crucial roles in physiological and pathological processes of life. In this paper, we review the recent research status of organic one and two-photon fluorescent probes for RSS in mitochondria, such as H2S, SO2, biothiols, H2Sn , etc. The properties of fluorescent probe selectivity, sensitivity, recognition mechanism, cell toxicity and biological monitoring imaging are emphatically discussed. Moreover, the existing problems and future development of this kind of fluorescent probes are proposed．This review is significantly meaningful in theory and practice for the construction of novel mitochondrial fluorescent probes and further dissection of the cellular function of RSS.