In this paper, taking triphenylamine as a raw material, 4,4'-(ethene-1,2-diyl)bis (N,N-diphenylaniline) (TPAS) has been synthesized, which was composed by C=C and two triphenylamine groups via Vilsmeier-Haack reaction and McMurry reaction. The optical performance of TPAS has been studied by UV-Vis absorbance spectrum and fluorescence spectrum. The results showed that TPAS had strong fluorescence emission, and the fluorescence emission was blue light. The fluorescence intensity had a good linear relationship with the concentration, and the solvation effect was not strong, and the optical stability was good. The aggregation of TPAS has been characterized by scanning electron microscope(SEM). And the results showed that when the volume fraction of water increased, the TPAS molecules accumulated and the morphology changed. The Cytotoxicity of TPAS was tested by MTT assays, the cell viability was above 80% with the increase of TPAS concentration in the text, which was revealed that TPAS is biocompatible and have low toxicity to cells. Then achieved imaging of A549.