In this study, lumpfish cartilage as raw material,The polysacccaride of Lumpfish cartilage was extracted by water extraction, and Sevag method was used to deproteinization. The purification of polysaccharide was carried by DEAE-52 cellulose and Sephadex G-75,after dialyzed and freezed，three component polysaccharides were obtained, the three polysaccharides composition were analyzed. Full-wavelength UV was used to scan, the heparin titer of the three polysaccharides was measured by the azure A colorimetric method. The structural of FCPS-3 was analysized by infrared and nuclear magnetic resonance, the FCPS-3 molecular weight was determined by GPC. The results showed that the polysaccharide content of all three components exceeded 90%, the protein content was less than 3%, and the uronic acid content exceeded 60%.The full-wavelength UV scanning of the three polysaccharides showed that the three polysaccharides had no characteristic absorption peaks at 260 nm and 280 nm, indicating that none protein and nucleic acids were contained in three polysaccharides.The heparin titer of FCPS-3 was the highest, 1.365 U/mg, and FCPS-1 and FCPS-2 were low. Characteristic showed FCPS-3 was acid polysaccharides and contained sulfate as β-glucopyranose. the FCPS-3 Mw was 175700 Da, the Z average molecular weight was 253032 Da, and the Mn was 121885 Da.When the concentration was 5 mg/ml, DPPH•, ABTS•, OH• free radical scavenging activity of the FCPS-3 was 78.63%, 96.26%, 93.15% , respectively.