文章摘要
柳仁民,刘晓燕.大孔吸附树脂纯化东北岩高兰总黄酮[J].精细化工,2019,36(4):
大孔吸附树脂纯化东北岩高兰总黄酮
Purification of total flavonoids from Empetrum nigrum L. var. japonicum with macroreticular resin
投稿时间:2018-06-13  修订日期:2018-12-11
DOI:
中文关键词: 东北岩高兰  总黄酮  大孔吸附树脂  纯化
英文关键词: Empetrum nigrum L. var. japonicum  total flavonoids  macroporous resin  purification
基金项目:国家自然科学基金项目(面上项目,重点项目,重大项目)
作者单位E-mail
柳仁民 聊城大学化学化工学院 renminliu@126.com 
刘晓燕 聊城大学化学化工学院  
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中文摘要:
      摘要:以东北岩高兰总黄酮的吸附和解吸能力为依据,采用静态吸附和解吸实验对8种型号的大孔吸附树脂进行筛选,结果显示AB-8型大孔吸附树脂对东北岩高兰总黄酮具有较好的吸附和解吸性能。进一步探究纯化工艺,确定最佳工艺条件为:静态吸附平衡时间为1.0 h,解吸溶剂为95%乙醇,静态解吸平衡时间为1.5 h,不同温度(25℃、30℃、35℃)下AB-8型大孔吸附树脂对东北岩高兰不同成分的吸附等温线均符合 Freundlich模型和Langmuir模型;最大上样体积为400 mL,5 BV 20%乙醇洗杂质,95%的乙醇洗脱成分,洗脱体积为5 BV,洗脱流速为3 mL/min。在最佳实验条件下东北岩高兰总黄酮的纯度由49.16%提高到了89.59%,为进一步研究东北岩高兰提供参考依据。
英文摘要:
      Abstract: Eight types of macroporous resins were compared via static adsorption-desorption methods with adsorption capacity and desorption capacity as dexes. Result indicated that AB-8 resin performed better adsorption and desorption properties to total flavonoids from Empetrum nigrum L. var. Japonicum. Then, the purification process was study by static and dynamic adsorption-desorption experiments. The optimal conditions were as follow: static adsorption equilibrium time was 1.0 h, desorption solvent was 95% ethanol and desorption equilibrium time was 1.5 h. Adsorption isotherm of five components from Empetrum nigrum L. var. japonicum by AB-8 macroporous adsorption resin according with Freundlich model and Langmuir model at different temperatures(25℃、30℃、35℃). The maximum sample volume was 400 mL, the impurities were washed by 5 BV 20% ethanol, and the components eluted with 95% ethanol, elution volume was 5 BV and elution flow rate was 3 mL/min. Finally, the purity of total flavonoids from Empetrum nigrum L. var. Japonicum increased from 49.16% to 89.59% at the optimal experimental conditions. This experiment provides a reference for further study of Empetrum nigrum L. var. japonicum.
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