文章摘要
李丽丽,刘雄民.柠檬桉树脂总黄酮的提取纯化及抗氧化活性[J].精细化工,2019,36(8):
柠檬桉树脂总黄酮的提取纯化及抗氧化活性
Extraction, Purification and antioxidant activities of total flavonoid in eucalyptus citriodira resin
投稿时间:2018-09-21  修订日期:2019-03-07
DOI:
中文关键词: 柠檬桉  树脂  总黄酮  萃取纯化  抗氧化活性  中药现代化技术
英文关键词: eucalyptus citriodira  resin  total flavonoid  purification  antioxidant activity  modernization technology of traditional Chinese medicines
基金项目:国家自然科学基金项目(面上项目,重点项目,重大项目)
作者单位E-mail
李丽丽 广西大学 ximengbali18@163.com 
刘雄民 广西大学 xmliu1@gxu.edu.cn 
摘要点击次数: 32
全文下载次数: 0
中文摘要:
      采用溶剂法提取纯化柠檬桉树脂总黄酮,对粗提物(A)和纯化物进行抗氧化活性研究。用盐酸-锌粉、浓硫酸、三氯化铁、硝酸铝等方法定性鉴别树脂总黄酮,以芦丁为标准品定量分析树脂总黄酮含量(以柠檬桉树脂固体粉末的质量为基准)为4.64%。粗提物(A)经氯仿-乙酸丁酯、氯仿-二甲基甲酰胺、二甲基甲酰胺-氯仿两次超声萃取后得到纯化物B、C、D中总黄酮质量分数分别为10.39%、18.03%、44.15%。考察了A、B、C、D对2,2-二苯基-1-苦味基肼自由基(DPPH·)的清除活性、总抗氧化能力和还原能力。结果表明,A、B、C、D对DPPH·的清除活性分别为91.75%、74.22%、88.44%、91.56%;总抗氧化能力分别为19.99、1.75、4.39、3.79 μmol 芦丁当量(RE)/g;还原能力分别为1.16、0.36、0.39、0.45μmol RE/g。
英文摘要:
      The total flavonoids in eucalyptus citriodira were extracted and purified by solvent extraction, and the antioxidant activity and purification of crude extract(A) were studied. Total flavonoids in resin were qualitatively identified by hydrochloric acid-zinc powder, concentrated sulfuric acid, ferric chloride and aluminum nitrate. Total flavonoids content(based on the quality of eucalyptus citriodira resin solid powder) was 4.64% by quantitating analysis with rutin as standard substance. B、C、Dpurifications of different purity were obtained by chloroform-butyl acetate, chloroform-dimethyl formamide, dimethyl formamide-chloroform ultrasonic extractions. The total flavonoid mass fraction of B, C, D were 10.39%, 18.03% and 44.15%.The scavenging activities of 2, 2-diphenyl-1-bitter hydrazine (DPPH·), total antioxidant capacity and reduction capacity of A, B, C, Dwere investigated. The scavenging activities of DPPH· were 91.75%, 74.22%, 88.44%, 91.56%; Total antioxidiant activities were 19.99, 1.75, 4.39, 3.79μmol rutin equivalent(RE)/g. The reducing abilities were 1.16,0.36,0.39,0.45μmol RE/g.
  查看/发表评论  下载PDF阅读器
关闭