In this work, nano-titanium dioxide (T-NTO) was prepared by hydrothermal synthesis using tannins as template. The structure and morphology of T-NTO were characterized by FT-IR, XRD, BET, SEM, TEM, and its adsorption performances for uranium were investigated. The results show that the specific surface area of T-NTO was significantly increased，and the adsorption capacity of the T-NTO exhibits obvious different varying with different tannins as template. It was found that the nano-titaniumdioxide (BT-NTO) prepared using bayberry tannin as template has the smallest particle size and its specific surface area is up to 96.61 m2／g. Furthermore, the adsorption process of uranium by T-NTO can be described by the Langmuir isothermal model and pseudo-second-order kinetic equation, and the adsorption capacity of BT-NTO to uranium is attained to 0.7054 mmol／g at 318K. In addition, the coexisting ions, including Zn2+, Mg2+, Pb2+, Cu2+, Mn2+, Na+, Cl-, SO42- and CO32- have little effect on the adsorption capacity of uranium by BT-NTO. However, the effect of F- is very significantly, but this effect can be greatly reduced by introducing Al3+. It should be noted that the BT-NTO can be regenerated with 0.1 mol／L HNO3 solution, and can be reused many times without obvious decreased of adsorption capacity.