文章摘要
张凯强.有机介质中包衣菌体催化合成cis9,trans11- 共轭亚油酸[J].精细化工,2019,36(8):
有机介质中包衣菌体催化合成cis9,trans11- 共轭亚油酸
Surfactant-coated bacteria as a catalyst for biosynthesis of cis9,trans11-conjugated linoleic acid in organic solvent
投稿时间:2019-01-12  修订日期:2019-03-28
DOI:
中文关键词: 共轭亚油酸  生物合成  包衣菌体  有机介质
英文关键词: conjugated linoleic acid  biosynthesis  surfactant-coated bacteria  organic solvent
基金项目:
作者单位E-mail
张凯强 中德联合研究院 790400754@qq.com 
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中文摘要:
      以三种脂肪醇正辛醇、月桂醇和肉豆蔻醇为原料,合成得到三种谷氨酸二烷基酯核糖醇(2C8GE、2C12GE和2C14GE),比较了不同谷氨酸二烷基酯核糖醇及其浓度和所用溶剂,以及缓冲液pH、包衣时间、温度和干燥方式对包衣Lactobacillus helveticus L7菌体酶活的影响。确定了制备包衣菌体的条件为:表面活性剂为谷氨酸二十二烷基酯核糖醇(2C12GE),配制成质量百分比1.0%(w/w)的丙酮溶液;菌体用pH5.8的磷酸盐缓冲液重悬;在4℃下处理4 h经冷冻干燥而成。在以亚油酸为底物时包衣菌体中亚油酸异构酶的Km为35.7 mM,Vmax为5.6 mM/h。经包衣处理后,菌体中亚油酸异构酶和底物的亲和力有所降低。
英文摘要:
      Three glutamic acid dialkyl ester ribitol (abbreviated as 2C8GE、2C12GE and 2C14GE) as the nonionic surfactants were synthesized with n-octyl alcohol, lauryl alcohol, and myristyl alcohol, respectively. Lactobacillus helveticus L7 with the ability to biosynthesize cis9,trans11-conjugated linoleic acid in aqueous was coated with these surfactants. Effects of the surfactants type, concentration, solvent, buffer pH, time, temperature and dry methods on the activity of enzyme in coated bacteria were studied. The optimized conditions were bacteria resuspended in pH5.8 buffer and coated with 1.0%(w/w) 2C12GE in acetone, incubated at 4℃ for 4 h, and lyophilized. The Km for lionleic acid as the substrate was 35.7 mM, and Vmax was 5.6 mM/h. The affinity of linoleic acid isomerase and linoleic acid was decreased as the bacteria were surfactant-coated.
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