文章摘要
方倩,彭志平.温度响应的酶催化核交联胶束[J].精细化工,2019,36(7):
温度响应的酶催化核交联胶束
Enzymatically Temperature-Responsive Core-Crosslinked Micelle
投稿时间:2019-01-20  修订日期:2019-03-12
DOI:
中文关键词: 核交联胶束  酶催化交联  聚(4-乙烯基苯酚)  温度响应  两亲性嵌段共聚物
英文关键词: core-crosslinked micelle  enzymactically crosslinked  poy(4--vinyl phenol)  temperature-responsive  amphiphilic block copolymer
基金项目:国家自然科学基金(21664010);江西省自然科学基金(20161BAB206121);南昌大学研究生创新专项资金(CX2017003)
作者单位E-mail
方倩 南昌大学 zzpeng@ncu.edu.cn 
彭志平 南昌大学 zzpeng@ncu.edu.cn 
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中文摘要:
      以对乙酰氧基苯乙烯(4-ASt)和N-异丙基丙烯酰胺(NIPAm)为单体,通过可逆加成-断裂链转移聚合(RAFT)合成了结构明确窄相对分子质量分布(Mw/Mn=1.28)的两嵌段共聚物P(4-ASt-b-NIPAm)。通过水合肼将P(4-ASt-b-NIPAm)中的P(4-ASt)嵌段上的乙酰基团选择性水解后得到聚(4-乙烯基苯酚-b-N-异丙基丙烯酰胺)两嵌段共聚物P(4-VPh-b-NIPAm)。采用1HNMR、FTIR和GPC对P(4-VPh-b-NIPAm)结构进行了表征,利用荧光光谱、透光率法和DLS研究了其水溶液的聚集行为和温度响应性。结果表明,P(4-VPh-b-NIPAm)在水中可自组装形成以(P4-VPh)为核、PNIPAm为壳的胶束,其临界胶束浓度(CMC)为0.015g/L,室温下平均粒径约为137 nm,低临界溶解温度(LCST)为31.2℃。以辣根过氧化物酶(HRP)催化交联P(4-VPh)核制备了平均粒径约为119 nm的核交联胶束,TEM显示干态核交联胶束为球形,粒径为60~100nm。核交联胶束水溶液具有良好的稳定性和温度响应性,其相转变温度范围为32~36℃,LCST约为34.9℃,随温度升高平均粒径从25℃的119nm减小至37℃的92.9nm。
英文摘要:
      Diblock copolymer P(4-ASt-b-NIPAm) with well-defined structure and narrow molecular-weight distri-bution (Mw/Mn=1.28) was synthesized by reversible addition-fragmentation transfer (RAFT) polymeriza-tion using poly(4-acetoxystyrene) P(4-ASt) and N-isopropylacrylamide (NIPAm) as monomers. The ace-tyl groups in the chain of P(4-ASt-b-NIPAm)) were selectively hydrolyzed by hydrazine hydrate to obtain diblock copolymer P(4-VPh-b-NIPAm). Copolymer P(4-VPh-b-NIPAm) was characterized by 1HNMR, FTIR and GPC. The aggregation behavior and temperature response of P(4-VPh-b-NIPAm) aqueous solu-tion were studied by fluorescence spectrum, transmittance method and DLS. The results showed that co-polymer P(VPh-b-NIPAm) could self-assemble into micelles using P(4-VPh) as core and PNIPAm as shell in aqueous solution and the critical micelle concentration (CMC) was 0.015 g/L. The average parti-cle size was about 137 nm at room temperature and the lower critical solution temperature (LCST) was 31.2℃. Horseradish peroxidase (HRP) catalyzed cross-linking of P(4-VPh) core to prepare core-crosslinked micelles with an average particle size of 119 nm. TEM showed that dry core-crosslinked micelles were spherical with particle size of 60~100 nm. The aqueous solution of core-crosslinked mi-celles had good stability and temperature response. Its phase transition temperature ranged from 32℃ to 36℃ and its LCST was about 34.9℃. The average diameter of core-crosslinked micelles decreased from 119 nm to 93 nm with increasing temperature from 25℃ to 37℃.
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