文章摘要
王幻,朱思明.橙皮苷酶解液中橙皮素单糖苷的树脂分离[J].精细化工,2019,36(8):
橙皮苷酶解液中橙皮素单糖苷的树脂分离
Separation of Hesperetin-7-O-glucoside from Hesperidin Hydrolysate
投稿时间:2019-01-29  修订日期:2019-03-22
DOI:
中文关键词: 橙皮苷酶解液  橙皮素单糖苷  分离纯化  大孔树脂  半制备液相
英文关键词: hesperidin hydrolysate  hesperetin-7-O-glucoside  separation and purification  macroporous resin  semi-preparative liquid chromatography
基金项目:国家自然科学基金项目(面上项目,重点项目,重大项目)
作者单位E-mail
王幻 华南理工大学 569417247@qq.com 
朱思明 华南理工大学 lfsmzhu@scut.edu.cn 
摘要点击次数: 33
全文下载次数: 0
中文摘要:
      橙皮苷酶解液组分复杂,含糖类、橙皮素和橙皮素单糖苷(hesperetin-7-O-glucoside,HMG)等。采用了6种大孔吸附树脂和半制备液相色谱分离纯化橙皮苷酶解液中的HMG,并通过红外、紫外和XRD手段表征产物结构,同时采用羟基自由基清除试验探讨其抗氧化活性。结果表明:HPD300大孔树脂最适于酶解液中HMG的分离;HPD300大孔树脂对酶解液中主要组分的静态和动态总吸附量分别为48.35 mg/g和35.82 mg/g,主要用于分离糖类杂质;以10 BV的45%乙醇为洗脱剂,洗脱流速1.2 mL/min,洗提物中HMG纯度从80.37%提高到97.83%;洗提物经半制备液相进一步分离纯化后得到单体HMG,最终纯度可达98.21%, 经紫外、红外和XRD手段验证产物为HMG;且HMG的羟基自由基清除能力略高于橙皮素,远高于橙皮苷。
英文摘要:
      The composition of hesperidin hydrolysate is complicated, including saccharides, hesperetin and hesperetin-7-O-glucoside(HMG). HMG from hesperidin hydrolysate was separated and purified by six macroporous adsorption resins and high performance semi-preparative liquid chromatography. The structure of the product was characterized by IR, UV-Vis and XRD analysis. And the antioxidant activities of HMG, hesperidin and hesperetin were compared by hydroxyl radical scavenging test. The results showed that HPD300 macroporous resin was the most suitable resin for the purification of HMG from enzymatic hydrolysis solution. The static and dynamic total adsorption amounts of HPD300 resin to the main components of enzymatic hydrolysis solution were 48.35 mg/g and 35.82 mg/g, respectively,which were mainly used for separation of sugar impurities. The purity of HMG in the eluent was increased from 80.37% to 97.83% by using 10BV, 45% ethanol as eluent with the flow rate of 1.2 mL/min. The final purity of monomer HMG, was 98.21% after further separation and purification by semi-prepared liquid phase. The product was verified as HMG by UV-Vis, IR and XRD spectra. And the hydroxyl radical scavenging capacity of HMG was slightly higher than those of hesperetin, much higher than that of hesperidin.
  查看/发表评论  下载PDF阅读器
关闭