文章摘要
张文.不同前处理对动物组织中氯丙嗪残留检测对比[J].精细化工,2019,36(8):
不同前处理对动物组织中氯丙嗪残留检测对比
Detection of chlorpromazine residues in animal tissues by different sample preparation methods
投稿时间:2019-03-21  修订日期:2019-05-07
DOI:
中文关键词: 氯丙嗪  液相色谱  质谱  前处理  固相萃取  QuECHERS  叔丁基甲醚
英文关键词: chlorpromazine  LC  MS  sample preparation  solid-phase extraction  QuECHERS  tert-Butyl methyl ether
基金项目:四川省科技厅应用基础研究计划项目( 2016JY0110);农业部农产品质量安全重点实验室开放课题(2018-KF-04)
作者单位E-mail
张文 西南科技大学 zhangwayne1988@163.com 
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中文摘要:
      采用超高效液相色谱串联质谱技术,比较QuECHERS法、MCX固相萃取法和叔丁基甲醚直接提取法3种前处理方法对猪肉、猪肝、鸡肉、鱼肉中氯丙嗪残留检测效果的影响。结果表明:3种方法对氯丙嗪的检出限为0.2 μg/kg,定量限为0.5 μg/kg。在不同加标水平下,QuECHERS法、MCX固相萃取法和叔丁基甲醚直接提取法处理的各类基质中加标回收率(75.5%~108.1%)和精密度(2.1%~7.5%)均满足方法学要求。MCX固相萃取法净化效果优于QuECHERS法、叔丁基甲醚直接提取法。整体回收率从高到低依次是MCX固相萃取法、QuECHERS法、叔丁基甲醚直接提取法。对于脂肪含量较高的猪肉,3种前处理方法回收率均略低于其他3种基质。
英文摘要:
      Ultra performance liquid chromatography tandem mass spectrum/ mass spectrum (UPLC-MS/MS) was used to detect chlorpromazine residue in porcine muscle, liver, chicken and fish. Three sample preparation methods, including QuECHERS, MCX solid-phase extraction, and direct extraction by tert-butyl methyl ether, were compared. The results showed that the limit of detection (LOD) of the three methods for chlorpromazine was 0.2 μg/kg, and the limit of quantitation (LOQ) was 0.5 μg/kg. The recovery and relative standard deviation (RSD) of the three sample preparation methods at different addition levels were 75.5%~108.1% and 2.1%~7.5%, respectively. The detection limit, recovery, and RSD meet the methodological requirements. The purifying effect of MCX solid-phase extraction was better than the other sample preparation methods. The order of recovery was as follows: MCX solid-phase extraction, QuECHERS, and direct extraction by tert-butyl methyl ether. The recoveries of all the three methods for porcine muscle were lower than that for liver, chicken and fish.
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