文章摘要
王卫强,崔静,吴尚书,李小玲,张海娟.复配菌对原油除蜡降黏效果分析[J].精细化工,2019,36(10):
复配菌对原油除蜡降黏效果分析
Analysis of compound bacteria on waxy crude oil
投稿时间:2019-04-01  修订日期:2019-05-16
DOI:
中文关键词: 复配菌  优化  原油  乳化  降黏  除蜡  菌株鉴定
英文关键词: compound bacteria  optimization  crude oil  emulsification  viscosity reduction  dewaxing  strain identification
基金项目:辽宁省高等学校创新人才( LR2018055);辽宁省自然科学基金指导计划(20180550745)
作者单位E-mail
王卫强 辽宁石油化工大学石油天然气工程学院 wwq920285@163.com 
崔静 辽宁石油化工大学石油天然气工程学院  
吴尚书 辽宁石油化工大学石油天然气工程学院  
李小玲 辽宁石油化工大学石油天然气工程学院  
张海娟 辽宁石油化工大学石油天然气工程学院 zhj_w@163.com 
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中文摘要:
      为提高含蜡原油流动性能,从辽河油田附近经受原油污染的土壤中筛选出两株原油降解菌。经过16S rRNA基因序列比对鉴定,菌株为纤维微菌属(Cellulosimicrobium sp.)和假单孢菌(Pseudomonas sp.),命名为7#和12#。将筛选出的7#菌与12#菌优化复配使用,研究结果表明:复配菌对液体石蜡有乳化效果,当复配菌体积比例为1:1时,乳化率最高达到77.5%;最适的生长温度为35~40℃,pH=6~8,最佳接种体积比为4%,复配体积比例为2:1,且培养基在初始pH,即pH=7时复配菌生长状态最佳。复配菌在37℃,pH=7条件下对原油处理7d后,除蜡率为61.32%,降黏率达到31.58%;显微镜观察蜡晶变小,进一步说明菌株能够降解石蜡,破坏蜡晶结构,提高含蜡原油的流动性能。
英文摘要:
      In order to improve the flow properties of waxy crude oil, two strains were isolated from the oil contaminated soil near Liaohe Oilfield, which can degrade crude oil. The strains were identified as Cellulosi microbium sp. and Pseudomonas sp., and named 7# and 12# by 16S rRNA gene sequence analysis. The results showed that the compound bacteria could emulsify the liquid paraffin; when the volume ratio of compound bacteria was 1:1, the highest emulsification rate reached up to 77.5%; the optimal temperature was 35~40℃; the pH was 6~8, and the best growth of the compound bacteria was at the initial pH; the optimal inoculum volume ratio was 4%, the volume ratio was 2:1. After the crude oil was treated for 7 days with the compound bacteria at 37℃ and pH=7, the wax removal rate was 61.32% and the viscosity reduction rate reached up to 31.58%. Through microscope observation, wax crystals became smaller, further indicating that the strain could degrade the wax, which improved the flow properties of waxy crude oil.
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