A high temperature activated industrial gelatin wood adhesive was synthesized via the reaction between gelatin and isocynate groups released by crosslinking agent, using industrial gelatin extracted from waste leather as raw materials and blocked waterborne polyurethane prepared by methyl ethyl ketoxime as crosslinking agent. FT-IR and DSC showed that the hydroxyl group on the methyl ethyl ketone oxime molecule was polycondensed with the isocyanate group on the polyurethane prepolymer, and the maximum unblocking temperature was about 126 °C. SEM showed that the surface of the adhesive was smoother after curing at high temperature, indicating that the compatibility between gelatin and the blocked waterborne polyurethane component was improved. The application test results show that the wet shear strength of adhesive is up to 0.97MPawhen the content of the blocked waterborne polyurethane is 20%of the mass of gelatin aqueous solution, which meets the requirements of the national class II plywood. The optimum hot pressing process conditions are a hot pressing temperature of 150℃, a hot pressing time of 4 min, and a coating amount of 300 g/m2. Keywords: industrial gelatin, blocked waterborne polyurethane, wood adhesive, shear strength Foundation item: National Natural Science Foundation of China(No. 21776169)