文章摘要
仇燕,杜星芳,贾艳丽.菜芙蓉多糖的表征与生物活性[J].精细化工,2020,37(6):
菜芙蓉多糖的表征与生物活性
Characterization and bioactivity of polysaccharide from Abelmoschus manihot (L.) Medic
投稿时间:2019-11-25  修订日期:2020-01-13
DOI:
中文关键词: 菜芙蓉  多糖  表征  生物活性  中药现代化技术
英文关键词: Abelmoschus manihot (L.) Medic  polysaccharide  charaterization  bioactivity
基金项目:河北省高等学校科学技术研究重点项目(ZD2018080);京津冀三地联合攻关项目(19YFSLQY00100)
作者单位E-mail
仇燕 河北科技大学生物科学与工程学院 qiuyan2015@126.com 
杜星芳 河北科技大学生物科学与工程学院  
贾艳丽 河北科技大学生物科学与工程学院  
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中文摘要:
      以菜芙蓉花醇提后的残留物为原料,通过水提醇沉法提取菜芙蓉多糖,测定多糖总糖含量及得率。经DEAE-52柱层析分离纯化菜芙蓉多糖〔Abelmoschus manihot (L.) Medic polysaccharide, AMP〕,确定AMP单糖组成,考察AMP的体外抗氧化能力,并对AMP保护质粒DNA及抑制肝癌细胞HepG2的增殖作用进行了考察。结果表明:菜芙蓉花多糖得率为13.47%,总糖含量为97.43%,AMP主要由鼠李糖、葡萄糖、阿拉伯糖、岩藻糖组成,其物质的量比为1.44∶5.44∶1.00∶5.37,体外清除自由基能力表现为O2-· > ABTS+· > ·OH > DPPH· > NO·,8 g/L AMP抑制H2O2诱导的DNA氧化损伤效果最好,在0.125~5.000?g/L范围内,随着AMP质量浓度的增加HepG2细胞的增殖抑制率逐渐增大,说明AMP具有抗肿瘤活性。
英文摘要:
      The polysaccharide was extracted from the ethanol-extracted debris of Abelmoschus manihot (L.) Medic flower by water extraction and alcohol precipitation method. The total sugar content and extraction rate were determined. The Abelmoschus manihot (L.) Medic polysaccharide (AMP) was obtained by purification through DEAE-52 column chromatography and monosaccharide composition of AMP were determined. The bioactivities including antioxidant activity in vitro, DNA protecting activity and hepatic cancer cell HepG2 growth inhibitory effect of AMP were investigated. The results showed that the extraction rate reached 13.47% and the content of total sugar was 97.43%. AMP was mainly composed of rhamnose, glucose, arabinose and fucose at a molar ratio of 1.44:5.44:1.00:5.37. The order of radicals scavenging?capacities?of AMP was O2-· > ABTS+· > ·OH > DPPH· > NO· , which suggested AMP had certain antioxidant?activity?in vitro. 8 g/L AMP had the best inhibition effect on DNA oxidative damage induced by H2O2 . The?inhibitory?ratio?of?HepG2 cell?increased with?the increase of AMP concentration ranging from?0.125?to?5.000 g/L, indicating?that?AMP?had potential?antitumor activity.
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