文章摘要
刘庆宇,姜娜.基于1,8-萘酰亚胺检测细胞内肼的荧光探针[J].精细化工,2020,37(8):
基于1,8-萘酰亚胺检测细胞内肼的荧光探针
A fluorescent probe based on 1, 8-naphthimide for detecting hydrazine in cell
投稿时间:2020-03-03  修订日期:2020-05-07
DOI:
中文关键词: 荧光  探针  萘酰亚胺    细胞
英文关键词: fluorescence  probe  naphthalimide  hydrazine  cell
基金项目:国家自然科学基金青年科学基金项目(2160020760);山东省自然科学基金(ZR2016BQ05)
作者单位E-mail
刘庆宇 济南大学 332965935@qq.com 
姜娜 济南大学 chm_jiangn@ujn.edu.cn 
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中文摘要:
      以对氨基酚和3-溴肉桂酸为原料,以4-溴-1,8-萘酰亚胺为荧光母体设计合成了一例用于检测细胞内肼的荧光探针NH1。该探针能够对肼产生特异性响应。利用1H NMR、MS对NH1进行了结构确证。在体外测试中,探针NH1最大发射波长处(λem=535 nm)的荧光强度随着肼含量的增加而逐渐增强,并且具有良好的线性关系(R2=0.99),检测限为0.23 μmol/L。该探针有着良好的选择性和抗干扰能力,在广泛pH范围内(4.5~10.5)均能够特异性检测肼。该探针能够染色到细胞内对细胞内的肼进行检测。
英文摘要:
      A novel fluorescence probe NH1 for detection hydrazine was synthesized based on 4- bromo-1, 8-naphthalimide, P-aminophenol and 3-bromocinnamic acid. The probe exhibited good specific response to hydrazine. The structure was confirmed by 1H NMR and MS. The fluorescence intensity at the maximum emission wavelength (λem = 535 nm) of the probe NH1 was increased with the addition of hydrazine. And it showed a good linear relationship. The linear correlation coefficient was R2 = 0.99. The detection limit was 0.23 μmol/L. In addition, the probe has good selectivity and anti-interference ability, and can adapt to a large pH range from 4.5 to 10.5. NH1 successfully stained the cells and detected hydrazine in cells.
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