文章摘要
靶向光敏剂NaYF4:Yb,Tm@ NaGdF4:Yb@TiO2@PEI-PAA-FA的制备和性能
Preparation and Properties of Targeting Photosensitizer NaYF4:Yb,Tm@NaGdF4:Yb@TiO2@PEI-PAA-FA
投稿时间:2017-11-07  修订日期:2018-01-15
DOI:
中文关键词: 上转换纳米粒子  二氧化钛  阿霉素  叶酸  药物缓释  光动力治疗  化疗
英文关键词: upconversion nanoparticle  titanium dioxide  doxorubicin  folic acid  drug release  photo dynamic therapy  chemotherapy
基金项目:浙江省公益技术应用研究计划项目(2016C31014)、浙江省自然科学基金(LY16H180002)、浙江师范大学“先进催化材料”教育部重点实验室和“固体表面反应化学”浙江省重点实验室开放课题基金(2017-15)、国家级大学生创新创业训练计划(201710345018).
作者单位E-mail
宫培军 浙江师范大学化学与生命科学学院 “先进催化材料”教育部重点实验室 skygpj@zjnu.cn 
毛艳 浙江师范大学化学与生命科学学院 “先进催化材料”教育部重点实验室  
梁天华 浙江师范大学化学与生命科学学院 “先进催化材料”教育部重点实验室  
胡小锋 浙江师范大学化学与生命科学学院 “先进催化材料”教育部重点实验室  
董孟杰 浙江大学医学院附属第一医院核医学科PET中心  
刘振锋 浙江大学医学院附属第一医院核医学科PET中心  
王瑶 浙江师范大学化学与生命科学学院 “先进催化材料”教育部重点实验室  
吴建卫 浙江师范大学化学与生命科学学院 “先进催化材料”教育部重点实验室  
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中文摘要:
      采用水解法对上转换纳米粒NaYF4:Yb,Tm@ NaGdF4:Yb包覆TiO2,然后修饰聚乙烯亚胺(PEI)和聚丙烯酸(PAA)并偶联叶酸(FA),制备了叶酸受体靶向纳米光敏剂(NaYF4:Yb,Tm@ NaGdF4:Yb@TiO2@PEI-PAA-FA)。借助XRD和TEM表征NaYF4:Yb,Tm@ NaGdF4:Yb@TiO2的物相和形貌,FTIR和ξ电位证实有机成分的修饰成功,并测试了产物的上转换发光光谱。在980 nm近红外光(NIR)下,纳米光敏剂存在下的1,3-二苯基异苯并呋喃溶液吸收光谱的降低证明了单线态氧的产生。此外,纳米光敏剂可以载带阿霉素(DOX),最大载药率为50.8%,包封率为84.7%。载药后的纳米光敏剂中DOX释放对介质具有pH响应性,在NIR照射的酸性介质(pH 5.0)中12 h的累积[累计还是累积?][累积]缓释率为38.1%,远高于中性介质(pH 7.4)的10.4%。结果表明,DOX负载和FA偶联的纳米光敏剂有望作为NIR驱动光动力治疗-化疗协同灭活肿瘤细胞的药物。
英文摘要:
      Folate receptor-targeted nanophotosensitizer (NaYF4:Yb,Tm@NaGdF4:Yb@TiO2 @PEI-PAA-FA) was prepared by coating TiO2 on the upconversion nanoparticles NaYF4:Yb,Tm@NaGdF4:Yb via a hydrolysis method, followed by modification with polyethyleneimine (PEI) and polyacrylic acid (PAA) and subsequent conjugation of folic acid (FA). XRD and TEM were used to characterize the crystal structure and morphology of the inorganic components of the nanophotosensitizer. FTIR and ξ potential were used to verify the binding of these organic components. Upconversion luminescence spectra of the samples were also measured. The decrease in the absorbance of 1,3-Diphenylisobenzofuran solution in the presence of the nanophotosensitizer under 980 nm near-infrared (NIR) laser irradiation indicated the singlet oxygen has been produced from the nanophotosensitizer. Besides, the photosensitizer has a doxorubicin (DOX) loading capacity, the maximum drug loading ratio was 50.8% and the encapsulation efficiency was 84.7%. The DOX release from the nanophotosensitizer was sensitive to the pH value of the buffer. As the DOX-loaded nanophotosensitizer was suspended in an acidic buffer of pH 5.0 and exposed to NIR laser irradiation, the cumulative release of DOX at 12 h was 38.1%, much higher than that obtained from a neutral buffer (pH 7.4), which was 10.4%. These results indicated the DOX-loaded and FA-coupled nanophotosensitizer may be a potential drug for synergistic inactivation of cancer cell by NIR-driven photodynamic therapeutic and chemotherapeutic modalities.
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