文章摘要
尿素-氯化胆碱低共熔体系提取羊毛角蛋白
Extraction of Wool Keratin by Urea-Choline Chloride Deep Eutectic Solvent
投稿时间:2018-06-21  修订日期:2018-10-02
DOI:
中文关键词: 羊毛角蛋白  尿素-氯化胆碱  低共熔体系  提取
英文关键词: wool keratin  urea-choline chloride  deep eutecticdeep eutectic solvent  extraction
基金项目:
作者单位E-mail
姜哲 江南大学生态纺织教育部重点实验室 1105338233@qq.com 
袁久刚 江南大学生态纺织教育部重点实验室 jiugangyuan@jiangnan.edu.cn 
王平 江南大学生态纺织教育部重点实验室  
范雪荣 江南大学生态纺织教育部重点实验室  
王强 江南大学生态纺织教育部重点实验室  
张连兵 江南大学生态纺织教育部重点实验室  
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中文摘要:
      以尿素-氯化胆碱低共熔体系为提取剂从羊毛纤维中提取羊毛角蛋白。最佳的条件为:溶解温度130 ℃,溶解时间180 min,m(羊毛)∶m(尿素-氯化胆碱低共熔体系)=1:33。采用FTIR,Raman,XRD,SDS-PAGE和TGA对原羊毛和再生羊毛角蛋白的结构进行了表征。结果表明:通过低共熔体系溶解再生得到的羊毛角蛋白,其主链结构得到了保留,但是与原羊毛相比,再生羊毛角蛋白中的α-螺旋结构数量减少,耐热性变差。
英文摘要:
      The urea-choline chloride deep eutectic solvent was applied to extract the wool keratin. The optimum conditions were dissolution temperature 130℃, dissolution time 180 min, and mass ratio of wool to urea-choline chloride deep eutectic solvent 1:33. FTIR, Raman, XRD, SDS-PAGE and TGA were used to characterize the structure of pristine wool and regenerated wool keratin. The results indicated the backbone of regenerated wool keratin was maintained during the process of dissolution. Compared with pristine wool fiber, the content ofα-helix structure in regenerated wool keratin was decreased, and the heat resistance was reduced.
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