文章摘要
罗丹明B衍生物荧光探针的合成及其对Fe3 的检测
Synthesis and Properties Characterization of Fe3+Fluorescent Probe Based on Rhodamine B
投稿时间:2018-10-15  修订日期:2019-01-31
DOI:
中文关键词: Fe(III)  荧光  检测  罗丹明B
英文关键词: Fe(III)  fluorescence  detection  Rhodamine B
基金项目:国家自然科学基金(No. 21703135)和陕西省自然科学基础研究计划(No. 2018JQ2070)资助项目
作者单位E-mail
延永 商洛学院 yananan@yeah.net 
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中文摘要:
      摘要:以罗丹明B与苯胺为原料,经过缩合反应制得一种罗丹明B衍生物的荧光探针F-1(3',6'-二(二乙基氨基)-2-苯基螺[异吲哚-3,9'-氧杂蒽]-1-酮),其结构通过1H NMR、13C NMR、红外光谱和高分辨质谱(HRMS)进行表征。Fe3+离子加入后探针体系后,体系颜色由色变为紫色,荧光显著增强,而不受的其他金属离子(Na+, K+, Ca2+, Mg2+, Zn2+, Hg2+, Co2+, Cd2+, Ni2+, Cu2+, Mn2+, Ag+, Pb2+, Cr3+, Fe2+)干扰。通过测定探针化合物F-1的离子选择性、离子竞争性、浓度梯度、Job曲线和pH对探针分子荧光强度的影响,发现在pH=5-7的环境中,探针F-1对Fe3+的检测在2.0×10-6~1.8×10-5 mol/L呈较好的线性关系,具备定性、定量检测生物机体或者环境中痕量浓度Fe3+的潜力。
英文摘要:
      Abstract: A probe base on Rhodamine B derivative F-1(3',6'-bis(diethylamino)-2-phenylspiro [isoindo-line-1,9'-xanthen]-3-one) has been synthesized and developed, which was prepared by condensation reac-tion of Rhodamine B with aniline. Its structure was characterized by 1H NMR, 13C NMR and HRMS. It was found that after the Fe3+ added, the probe system changed from colorless to purple and the fluores-cence intensity was significantly enhanced, while other metal ions (Na+, K+, Ca2+, Mg2+, Zn2+, Hg2+, Co2+, Cd2+, Ni2+, Cu2+, Mn2+, Ag+, Pb2+, Cr3+, Fe2+) were not disturbed. By measuring the ion selectivity, ion competi-tiveness, concentration gradient, Job curve, and the effect of pH on the fluorescence intensity of probe compound F-1, we found that probe compound F-1 presents a good linear relationship in pH=5-7 (2.0×10-6~1.8×10-5 mol/L), and has the potential of qualitative and quantitative detection of trace Fe3+ in bio-logical organisms and environment.
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