文章摘要
深共熔溶剂-水混合物同步提取红景天苷和酪醇的响应面优化及其分离回收
Simultaneous Extraction of Salidroside and Tyrosol Using Deep Eutectic Solvent-Water Mixture: Response Surface Optimization and Separation
投稿时间:2018-10-18  修订日期:2018-12-30
DOI:
中文关键词: 红景天苷  酪醇  深共熔溶剂  响应面法  大孔树脂  提取  回收
英文关键词: Salidroside  Tyrosol  Deep eutectic solvent  Response surface methodology  Macroporous resin  Extraction  Recovery
基金项目:
作者单位E-mail
苏二正 南京林业大学 ezhsu@njfu.edu.cn 
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中文摘要:
      以深共熔溶剂-水混合物(LAEG40)作为提取溶剂,红景天苷提取率为主要指标,酪醇提取率为辅助指标,在前期单因素实验的基础上,利用响应面法考察了液料比、提取温度、提取时间三个主要因素对红景天中红景天苷和酪醇同步提取的影响。得到的最佳提取条件为: 液料比12.05:1(mL/g),提取温度60 ℃,提取时间35 min。在此条件下LAEG40对红景天苷的提取率可达到19.3552?0.6604 mg/g,酪醇提取率可达到1.7211?0.0585 mg/g,远高于传统溶剂水、乙醇对红景天苷和酪醇的提取率。为实现LAEG40提取红景天苷和酪醇的回收,进行了大孔树脂吸附分离的研究。经过一系列优化,获得的最佳吸附分离条件为:选用SP-825树脂装柱,每10g树脂上样30 mL LAEG40提取液,洗脱剂选用80 %乙醇,洗脱体积40 mL,洗脱流速1 mL/min,在此条件下红景天苷和酪醇的回收率分别可达60.47 %和85.07 %。
英文摘要:
      Taking deep eutectic solvent-water mixture (LAEG40) as extraction solvent, extraction yield of salidroside as main index, and extraction yield of tyrosol as auxiliary index, the effects of three main parameters solvent to solid ratio, extraction temperature and extraction time on the simultaneous extraction of salidroside and tyrosol from Rhodiola rosea L were optimized by response surface methodology. The optimal extraction conditions were obtained as follows: solvent to solid ratio of 12.05:1 (mL/g), extraction temperature of 60 ?C and extraction time of 35 min. Under these conditions, the extraction yield of salidroside reached 19.3552?0.6604 mg/g, and the extraction yield of tyrosol reached 1.7211?0.0585 mg/g, which were much higher than those of traditional solvents (water and ethanol). In order to recover the salidroside and tyrosol from the LAEG40 extraction solution, the adsorption and separation method with macroporous resin was carried out. After systematical optimization, the optimal conditions for adsorption and separation were obtained as follows: SP-825 as the adsorption resin, 30 mL LAEG40 extraction solution to 10 g SP-825, 80 % ethanol as the elution solvent, eluting volume of 40 mL, and volume flow at 1mL/min. Under these conditions, the recovery rates of salidroside and tyrosol attained to 60.47 % and 85.07 %, respectively.
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