文章摘要
野棉花化学成分及抗肿瘤活性研究
Study on chemical constituents and antitumor activity of Amenone vitifolia
投稿时间:2019-04-15  修订日期:2019-07-23
DOI:
中文关键词: 野棉花  化学成分  抗肿瘤活性
英文关键词: Anemone vitifolia  constituents  anti-tumor activity
基金项目:国家自然科学基金项目(面上项目,重点项目,重大项目)
作者单位E-mail
刘雨 湖北民族大学 724785476@qq.com 
田从魁 湖北民族大学 pkutianck@163.com 
摘要点击次数: 36
全文下载次数: 0
中文摘要:
      采用硅胶、Sephadex LH-20、制备液相等色谱方法对恩施地区银莲花属植物野棉花体积分数为70%的乙醇提取液进行分离纯化,运用NMR、MS等方法进行结构鉴定,并考察了化合物的抗肿瘤活性。结果显示:得到了10个化合物,包括木脂素类、黄酮类、皂苷类化合物。分别为络石苷元(Ⅰ),牛蒡子苷元(Ⅱ),绿原酸(Ⅲ),紫云英苷(Ⅳ),异槲皮素苷(Ⅴ),银椴苷(Ⅵ),络石苷(Ⅶ),牛蒡子苷(Ⅷ),水杨苷(Ⅸ),薯蓣皂苷(Ⅹ)。其中化合物Ⅰ~Ⅱ、Ⅵ~Ⅶ、Ⅸ是首次从银莲花属中分离得到,化合物Ⅰ~Ⅶ、Ⅸ、Ⅹ是首次从野棉花中分离得到。MMT(四唑盐比色法)测试结果显示:化合物Ⅱ、Ⅷ、Ⅸ、Ⅹ在100 μmol/L浓度时对宫颈癌细胞HeLa的抑制率分别为10.5% , 7.0%, 0.5%及88.0%,其中化合物X的活性最好,高于阳性对照品顺铂的抑制率80.5%。
英文摘要:
      The 70% ethanol extract of Anemone vitifolia?in Enshi area of HuBei province was isolated?and purified by?column chromatography on?silica?gel, Sephadex?LH-20 and preparative liquid chromatography. Structures were proved by physicochemical properties, NMR and MS.The antitumor?activity?was tested by MTT assay. Results 10 compounds, including lignin, flavonoidsand saponins, were obtained from 70% ethanol extract of Anemone?vitifolia?, and were identified trachelogenin (Ⅰ),?arctigenin (Ⅱ),?chlorogenic?acid (Ⅲ),?astragalin (Ⅳ),?isoquercitrin (Ⅴ),?tiliroside (Ⅵ),?tracheloside (Ⅶ),?arctiin (Ⅷ),?salicin (Ⅸ)?and?dioscin (Ⅹ). CompoundsⅠ~Ⅱ、Ⅵ~Ⅶ、Ⅸ?were?isolated?from?the?genus?Amenone?for?the?first?time;?Compounds?Ⅰ~Ⅶ、Ⅸ、Ⅹ?were?isolated? from?Amenone?vitifolia?for?the?first?time. Compounds Ⅱ、Ⅷ、Ⅸ、Ⅹ inhibited the proliferation of HeLa cells with inhibition rates of 10.5%, 7.0%, 0.5% and 88.0% at 100 μmol/L, and the inhibition rate of compound Ⅹ was higher than that of the positive control cisplatin by 80.5%.
View Fulltext   查看/发表评论  下载PDF阅读器
关闭