In this study, dopamine and UiO-66 nanoparticles were co-deposited on polyetherimide (PEI) base membrane crosslinked by 1,6-hexamethylenediamine to construct PDA-UiO-66 nanocomposite interlayer, and the solvent-resistant composite nanofiltration membrane (TFN-U) was prepared by interfacial polymerization on the interlayer. The membrane structure was characterized and analyzed by FTIR, XRD, SEM, AFM, water contact angle meter, etc. Meanwhile, the influence of UiO-66 mass concentration on the solvent resistance, pollution resistance and operation stability of TFN-U membrane was explored. The results show that the introduction of PDA-UiO-66 nanocomposite interlayer can improve the permeation flux of TFN-U membrane. When the mass concentration of UiO-66 nanoparticles is 0.2 g/L, the pure water flux of TFN-U2 membrane is increased to 63.83 L/(m2 h) and the methanol flux is 28.50 L/(m2 h), and the rejection rate of Congo red aqueous solution and Congo red methanol solution can still maintain 98.24 and 93.16%. After soaking in anhydrous ethanol, acetone, ethyl acetate, n-hexane and DMF for 24 hours, the rejection rate of Congo red remained above 94%, and the flux recovery rate reached 78.1%. After continuously filtering Congo red methanol solution for 24 hours, the methanol flux of the membrane was 14.13 L/(m2 h) and the rejection rate was 98.29%, which indicated that TFN-U2 membrane had good solvent resistance, pollution resistance and certain stability.