The reducing end of high amylose starch (HS) is modified with magnet particles by "Borch reduction -Click chemistry" to obtain modified starch with magnetic properties and prepare a regenerated starch film under the external magnetic field. The experiment includes the following steps， alkyne groups are introduced into unique reductive end of HS molecule, at the same time, Fe3O4 magnet nanoparticles(MNP) are coated with silane to obtain azidated magnet particles(azide-MNPs). Finally, the modified starch (MMS) with magnetic terminal is obtained through Click chemistry reaction. The successful formation of MMS was confirmed by XRD, FTIR, NMR and MALDI-TOF MS, etc. Furthermore, the mechanical properties of the regenerated starch film were tested, and the results showed that MMS gradually tended to be arranged in an orderly manner under the conditions of magnetic field induction. The resulting regenerated starch film had a increased breaking elongation and breaking strength, and improved short-range order. This study provides a new idea for the structural design and mechanical properties control of starch regeneration membranes based on an external magnetic field.