A emulsion liquid membrane method with trioctylamine as carrier is used to extract 6-APA (6-Aminopenicillanicacid) from the wastewater. The influences of types of membrane solvents, carrier concentra-tion, internal phase concentration, oil/internal phase ratio (the volume ratio of oil to internal phase), the pH of external phase, emulsion/external phase ratio (the volume ratio of emulsion phase include internal aqueous phase to the external aqueous solution) and stirring speed on the transition of 6-APA are investigated. The results show that the extraction rate of 72.9% was achieved through one stage of emulsion liquid membrane separation in 30 min at conditions as: the volume ratio of kerosene, trioctylamine, liquid wax, surfactant Span80 were 88%, 5%，1%，6% , the mass concentration of Na2CO3 is 5%, V(oil phase):V(internal phase)= 2:1, the pH of external phase is 5.5～6.5, V(emulsion phase):V(external phase)=1:4, and the stirring speed is 300～350 r/min. Over two stage emulsion liquid membrane separation, the extraction rate can up to 93%. The centrifugal emulsion method was used to separate the oil phase and internal phase. The breaking rate of 65.2% was obtained. the recovery of oil phase was reused in emulsification, and the extraction of 6-APA can up to 68%. It is a technological method with high efficiency and environmental protection.