The molecularly imprinted microspheres for crystal violet (CV) were synthesized by precipitation polymerization in which crystal violet was used as template, α-methacrylic acid (MAA) as functional monomer and ethylene glycol dimeth acrylate (EGDMA) as crosslinker. Meanwhile, the properties of microspheres for different crosslinkers and dosage, different mole ratio of functional monomers to templates were also investigated. The optimal conditions were as follows: shaking bath, 30 mL acetonitrile as solvents, EGDMA as cross-linker and the molar ratio of CV : MMA : EGDMA= 1 : 4 : 20. Under this synthesis conditions, the adsorption capacity of polymer microspheres were as high as 39.1 μmol/g，separation factor α and imprinting efficiency factor β are 3.76 and 3.18 respectively. It showed good identification and adsorption ability to target molecules.