A temperature-sensitive glucose random polymer p (diethyleneglycol methacrylate) -co-p ( 6-O-vinyladipoyl-α-D-galactose) P(DEGMA-co-OVNGlu) was prepared by enzymatic reaction and reversible addition-fragmentation chain transfer (RAFT) polymerization using 6-O-vinyladipoyl-α-D-galactose (OVNGlu) and thermoresponsive diethyleneglycol methacrylate (DEGMA) as raw materials. The polymer structure and glycopolymer-lectin (Con A) mutual recognition were studied by means of Fourier transform infrared spectroscopy (FTIR), nuclear magnetic resonance spectroscopy (1H NMR), gel permeation chromatography (GPC), ultraviolet spectrophotometer (UV-Vis) and dynamic light scattering (DLS). The results showed that P(DEGMA-co-OVNGlu) was successfully prepared. Ultraviolet and dynamic light scattering results show that the random polymer containing glucose has certain recognition ability for Con A， with the extension of the conjugation time, the recognition of the glycopolymer and lectin reached saturation at 20 min, and then gradually formed cross-link.