文章摘要
朱劼,胡佳慧,杨坤,卢晓雪.病毒样颗粒限域纳米催化剂提高催化加氢活性[J].精细化工,2019,36(11):0
病毒样颗粒限域纳米催化剂提高催化加氢活性
Virus-like particle Confined Nanocatalyst Improving Activity in Catalytic Hydrogenation
投稿时间:2019-03-21  修订日期:2019-05-05
DOI:
中文关键词: 病毒样颗粒  豇豆褪绿斑驳病毒  原核表达  纳米催化剂  催化技术
英文关键词: Virus-like particle  Cowpea Chlorotic Mottle Virus  Prokaryotic Expression  Nanocatalyst  Catalysis technology
基金项目:国家自然科学基金项目(面上项目,重点项目,重大项目)
作者单位E-mail
朱劼 常州大学制药与生命科学学院 zhujie@cczu.edu.cn 
胡佳慧 常州大学制药与生命科学学院  
杨坤 常州大学制药与生命科学学院  
卢晓雪 常州大学制药与生命科学学院  
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中文摘要:
      以豇豆褪绿斑驳病毒(CCMV)衣壳蛋白表达基因为模板,构建基因工程菌CCMV-BL21,并通过正交实验优化可溶蛋白表达工艺;接着采用原位还原法制备病毒样颗粒限域Ru纳米催化剂(Ru@CCMV),以肉桂醛和4-硝基苯酚加氢为模型反应进行评价。目标蛋白的最佳表达条件为:诱导温度25 ℃,IPTG终浓度1 mmol/L,诱导时间15 h。在此条件下,可溶性蛋白表达量达181 mg/L发酵液,约占表达蛋白总量的93%。Ru@CCMV在两个反应中均显示较高的催化活性,反应速率常数经计算分别达到0.17 min-1和0.50 h-1,高于柠檬酸修饰Ru纳米颗粒(Ru-CA)催化剂(0.11 min-1和0.36 h-1)。
英文摘要:
      A genetically engineered bacteria CCMV-BL21 was firstly constructed, using the gene expressing cowpea chlorotic mottle virus (CCMV) capsid protein as the template. The expression process of soluble CCMV CPs was then optimized by orthogonal experiments. After that, Ru nanoparticles was encapsulated in CCMV CPs self-assembled virus-like particles (VLPs) by in-situ reduction. The prepared catalyst Ru@CCMV was evaluated in catalytic hydrogenation of cinnamaldehyde (CAL) and 4-nitrophenol (4-NP). Results showed that the optimal expression conditions of CCMV CPs were as follows: induction temperature 25 ℃, final concentration of IPTG 1 mmol/L and induction time 15 h. Under the optimal conditions, the content of expressed soluble CCMV CPs reached 181 mg/L fermentation broth, accounting for 93% of the total. Ru@CCMV exhibited higher catalytic activities in both reactions in comparison to Ru catalyst stabilized with citric acid ligands (Ru-CA) did. The reaction rate constants in hydrogenation of CAL and 4-NP over Ru@CCMV were calculated to be 0.17 min-1 and 0.50 h-1, respectively, while those over Ru-CA were only 0.11 min-1 and 0.36 h-1.
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