文章摘要
李维新,董爱文.响应面法优化管萼山豆根总生物碱提取工艺[J].精细化工,2019,36(11):
响应面法优化管萼山豆根总生物碱提取工艺
Optimization of Extracting Process for Alkaloid from Euchresta tubulosa Dunn by Response Surface Methodology
投稿时间:2019-04-18  修订日期:2019-05-31
DOI:
中文关键词: 管萼山豆根  总生物碱  微波辅助超声提取  抗氧化活性  中药现代化技术
英文关键词: Euchresta tubulosa Dunn  alkaloid  microwave-assisted ultrasound  antioxidant activity  modernization technology of traditional Chinese medicines
基金项目:国家自然科学基金项目(面上项目,重点项目,重大项目);吉首大学校级课题
作者单位E-mail
李维新 吉首大学林产化工工程湖南省重点实验室 1650259583@qq.com 
董爱文 吉首大学林产化工工程湖南省重点实验室 daw1999@163.com 
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中文摘要:
      采用微波控温辅助超声波提取管萼山豆根茎中总生物碱,通过单因素法和响应面法优化了提取工艺,考察了管萼山豆根茎中总生物碱对1,1-二苯基-2-三硝基苯肼自由基(DPPH?)、2,2-联氮-二-3-乙基-苯并噻唑-6-磺酸自由基(ABTS?)和超氧阴离子自由基(O2-?)清除作用。结果表明,模型优化并修正的最佳工艺条件为:提取时间37.8min,微波加热温度64.5℃,超声功率575W,乙醇体积分数95%,在此条件下总生物碱得率为0.5436%,高于不采用微波超声辅助0.2436%、超声辅助0.4538%、乙醇回流0.3884%。管萼山豆根茎中总生物碱在DPPH?、ABTS?、邻苯三酚浓度分别为0.1、7.0、8.0mmol/L条件下,其半数抑制质量浓度(IC50)为0.891、0.552和0.390g/L。
英文摘要:
      Microwave-assisted temperature-controlled ultrasonic extraction was used to extract total alkaloids from stem of Euchresta tubulosa Dunn. The extraction process was optimized by single factor method and response surface methodology. The scavenging effects of total alkaloids on 1,1-diphenyl-2-trinitrophenylhydrazine (DPPH?), 2,2-diazo-2-3-ethyl-benzothiazole-6-sulfonic acid (ABTS?) and superoxide anion (O2-?) free radicals were investigated. The results showed that the optimum conditions were as follows: extraction time 37.8 min, microwave heating temperature 64.5℃, ultrasonic power 575 W and ethanol volume fraction 95%. Under these conditions, the yield of total alkaloids was 0.5436%, higher than no microwave-assisted ultrasound 0.2436%, ultrasound assisted 0.4538% and ethanol reflux 0.3884%. When the concentration of the extracted total alkaloids was 0.1, 7.0 and 8.0 mmol/L in DPPH?, ABTS? and benzene-1,2,3-triol, respectively, the corresponding half maximal inhibitory mass concentrations (IC50) were 0.891, 0.552 and 0.390 g/L.
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