中间苍白杆菌对含蜡原油除蜡降粘分析
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TE832

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辽宁省自然科学基金指导计划(20180550745);辽宁省高等学校创新人才支持计划(LR2018055)


Analysis on Wax Removal and Viscosity Reduction of Waxy Crude Oil With Ochrobactrum intermedium
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    摘要:

    蜡质不仅使含蜡原油黏度升高,而且会析出积聚在管壁上降低管输效率。从原油污染淤泥中分离出一株产表面活性剂的烃降解菌F-1,经16S rDNA鉴定为中间苍白杆菌。与原油作用7 d 后,能将蜡含蜡量为15.2%的高含蜡原油处理为蜡含蜡量为9.1%的含蜡原油,原油蜡质量分数降低40.1%,原油黏度降低21%以上,细胞疏水性达28.1%。通过傅里叶红外光谱鉴定该菌产生的生物表面活性剂为脂肽类。添加该菌上清液到液体石蜡 可形成91.49 mm的排油圈,能够显著降低培养基表面张力,对液体石蜡乳化系数达到65%。

    Abstract:

    Waxy not only increases the viscosity of waxy crude oil, but also precipitates and accumulates on the pipe wall, reducing the efficiency of pipe transportation. A strain of surfactant producing hydrocarbon degrading bacteria F-1 was isolated from crude oil contaminated sludge and identified as Ochrobactrum intermedium by 16S rDNA. After the interaction with crude oil for 7 days, the high-wax crude oil with a wax content of 15.2% could be treated with a wax content of 9.1%, the crude oil wax mass fraction decreased by 40.1%, the crude oil viscosity decreased by more than 21%, and the cell surface hydrophobicity reached 28.1%. The biosurfactant produced by the bacteria was identified as lipopeptide by Fourier infrared spectroscopy. It can form a 91.49mm oil drainage ring, which can significantly reduce the surface tension of the medium and reach 65% emulsification coefficient of the liquid paraffin.

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冯宪明,马火宝,杜胜男,李承泽,王卫强.中间苍白杆菌对含蜡原油除蜡降粘分析[J].精细化工,2020,37(9):

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  • 收稿日期:2020-05-07
  • 最后修改日期:2020-06-03
  • 录用日期:2020-06-12
  • 在线发布日期: 2020-08-05
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