文章摘要
冯宪明,马火宝,杜胜男,李承泽,王卫强.中间苍白杆菌对含蜡原油除蜡降粘分析[J].精细化工,2020,37(9):
中间苍白杆菌对含蜡原油除蜡降粘分析
Analysis on Wax Removal and Viscosity Reduction of Waxy Crude Oil With Ochrobactrum intermedium
投稿时间:2020-05-07  修订日期:2020-06-03
DOI:
中文关键词: 中间苍白杆菌  生物表面活性剂  脂肽  含蜡原油  流动性  油田化学品
英文关键词: Ochrobactrum intermedium  biosurfactant  lipopeptide  waxy crude oil  liquidity  oil field chemicals
基金项目:辽宁省自然科学基金指导计划(20180550745);辽宁省高等学校创新人才支持计划(LR2018055)
作者单位E-mail
冯宪明 辽宁石油化工大学石油天然气工程学院 fxm8886@126.com 
马火宝 中海石油(中国)有限公司天津分公司渤南作业公司  
杜胜男 辽宁石油化工大学石油天然气工程学院 dusn808@163.com 
李承泽 辽宁石油化工大学石油天然气工程学院  
王卫强 辽宁石油化工大学石油天然气工程学院  
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中文摘要:
      蜡质不仅使含蜡原油黏度升高,而且会析出积聚在管壁上降低管输效率。从原油污染淤泥中分离出一株产表面活性剂的烃降解菌F-1,经16S rDNA鉴定为中间苍白杆菌。与原油作用7 d 后,能将蜡含蜡量为15.2%的高含蜡原油处理为蜡含蜡量为9.1%的含蜡原油,原油蜡质量分数降低40.1%,原油黏度降低21%以上,细胞疏水性达28.1%。通过傅里叶红外光谱鉴定该菌产生的生物表面活性剂为脂肽类。添加该菌上清液到液体石蜡 可形成91.49 mm的排油圈,能够显著降低培养基表面张力,对液体石蜡乳化系数达到65%。
英文摘要:
      Waxy not only increases the viscosity of waxy crude oil, but also precipitates and accumulates on the pipe wall, reducing the efficiency of pipe transportation. A strain of surfactant producing hydrocarbon degrading bacteria F-1 was isolated from crude oil contaminated sludge and identified as Ochrobactrum intermedium by 16S rDNA. After the interaction with crude oil for 7 days, the high-wax crude oil with a wax content of 15.2% could be treated with a wax content of 9.1%, the crude oil wax mass fraction decreased by 40.1%, the crude oil viscosity decreased by more than 21%, and the cell surface hydrophobicity reached 28.1%. The biosurfactant produced by the bacteria was identified as lipopeptide by Fourier infrared spectroscopy. It can form a 91.49mm oil drainage ring, which can significantly reduce the surface tension of the medium and reach 65% emulsification coefficient of the liquid paraffin.
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