Colloidal dispersion of indium tin oxide (ITO) nanoparticles in an butyl acetate solvent was achieved using a milling process in which PVP and Titanate as dispersants. The effect of various parameters such as different PH value, dispersant dosage, zirconium oxide ball diameter and interfaces and milling time factors on dispersion stability was studied, and the dispersing mechanisms on stability of ITO suspension which about the two kinds of dispersant were investigated. The optimum conditions obtained from the tests were that when the PH value of 7.8, dispersant dosage relative to 5.5%, the size of ZrO2 beads is no more than 0.5mm and the quantity of beads was determined to be 2.6 times weight of ITO, shearing time was 24h. Compare with the two kinds of ITO dispersant slurry prepared in the storage time of 0 days and 30 days of the slurry particle size changes, the stability of the latter than the former