In this paper, the main functional components was extracted from medlar leaves of Qaidam Basin by high pressure homogenization method and the extract was purified at the optimum condition. The extraction condition was optimized by orthogonal experiment on four simple factors such as ethanol concentration, solid-liquid ratio, extraction pressure, and extraction time. The optimum condition were as follow: ethanol concentration was 80%, solid-liquid ratio was 1:10, extraction pressure was 60MPa, extraction time was 30min. Under the method, rutin content of the extract was 10.53% and total flavonoids content was 32.61%. The condition of purification process was selected AB-8 macroporous resin and some parameters were decided in purification technology that the volume of water 5 BV, the concentration of used ethanol gradient and the adding volume 4 BV. Rutin content of purifications was up to 60.85% and total flavonoids content was up to 90.53% in these conditions.