The glycoside-based trisiloxane surfactant which has gathered the lower surface tension of trisiloxanes and the better biological degradability of carbohydrate-based surfactants was synthesized in term of the theory of the relationship of surfactant structure and property. The surface activity and moisturization properties of the surfactant in cosmetics were studied by determining the surface tension, critical aggregation concentration, aggregation size and moisturization. The results showed that the glycoside-based trisiloxane surfactant reduced the surface tension of water to approximately 20 mN/m. The critical aggregation concentration was a level of 10-4 mol/L. The average hydrodynamic radius of the surfactant aggregate in aqueous solution was about 250 nm. The skin moisture content of 33.36, hydration rate of 31.97%, skin moisture loss of 11.21, moisture loss rate of -11.54% are obtained using cosmetics with the glycoside-based trisiloxane in 2 h when the content of glycoside-based trisiloxane surfactant is 3% in the cosmetics. Excellent moisturizing effect of glycoside-based trisiloxane surfactant can supply hydration and hold water for the skin, allowing skin to be long-lasting moisturized and delicate.