An isatin Schiff base derivative, 3-((2-hydroxynaphthyl)methyl)hydrazono- indilin-2-one(HNHI) was designed and synthesized. Its structure was characterized by 1H NMR、13C NMR and element analysis. The influences of different metal ions on its UV-Vis absorbtion spectra and fluorescence spectrum were studied. The results showed that the absorption spectra of HNHI had good response to Cu2 , Co2 and Ni2 , and appeard a new peak in the long wavelength region. Then the solution was turned to pink from colorless. Besides, the fluorescence spectrum of HNHI had good selectivity to Cu2 , Co2 and Ni2 with obvious fluorescence enhancement.