A kind of anionic polymerizable lipids sodium conjugated linoleates was prepared from high purity natural linoleic acid through alkali catalytic isomerization reaction. The sodium conjugated linoleates molecularly self-assembled into spherical micelles in aqueous phase, and the intramicellar self-crosslinking reaction among the sodium conjugated linoleates were induced by some free radical initiators to result in formation of spherical self-crosslinking micelles of sodium conjugated linoleates. The influence such as type and amount of initiators, reaction times and temperatures were investigated on the self-crosslinking degree of the sodium conjugated linoleates and the morphologies of the self-crosslinking assemblies were intuitively imaged by TEM. Experimental results indicated that the self-crosslinking spherical micelles of 20 nm in average diameter were obtained when self-crosslinking was induced by using ammonium persulfate as initiator at pH 13 and 80 oC for 10 h, which is a potential stable drug nanocarrier or microreactor.