Kinds of 1,4 - disubstituted D-Glucosamine urea derivatives were synthesized from 1,3,4,6-tetra-O-benzyl-β-D-glucosamine hydrochloride, solid phosgene and hydrazine to demand for new compounds containing semicarbazide glucosamine molecule fragments. The method used in the experiments have characteristics of mild conditions, short reaction time, simple operation, easy post-processing, high yield and so on. For the synthesis of varieties of the representative target products, the method had good adaptability. Used acetyl hydrazide as the model to sort out the experimental conditions. The optimum conditions obtained by optimization of experimental conditions. The target products were synthesized in high yield with low toxicity and available materials. A convenient method was provided for the synthesis of urea derivatives containing glucosamine molecule fragment. The structure of target products were confirmed by IR spectroscopy, 1HNMR and ESI-MS spectra.