In order to make the xanthine oxidase (XOD) to be recoverable and durable, a new kind of immobilized XOD was prepared. The silica microspheres were chosen as the supporter and the carbon nanotubes (CNTs) were coated on the surface of microspheres by covalent bonding. Then, free XOD were immobilized on the surface of XOD@CNTs@SiO2. The morphology and chemical group on the new XOD@CNTs@SiO2 were characterized by TEM and FTIR, respectively. Uric acid, the reaction product, was used as the evaluation indicator of the relative activity. The loading of XOD was calculated by Bradford method. It was shown that, the loading and relative activities of XOD@CNTs@SiO2 were increased 2 and 2.6 times than XOD@SiO2. The relative activity could keep 80% after saving for 400 min under 60oC. The XOD@CNTs@SiO2 were kept under 4 oC and away from light, and the relative activity could still keep 60%-70% until the 11th day. Under the normal temperature, the relative activity could keep 66.1% after 9 times continuous reactions.