Abstract: The method of extraction and purification of 3 nonionic surfactants from Camellia Oleifera Abel meal，Sapindus Mukorossi Gaertn and Gleditsia Sinensis Lam were researched respectively，in addition，the influencing factors of their surfactant activity and their complex synergism were evaluated as well .The experimental results show that，after extraction and purification，the surfactant activity of the final extracts were obviously superior to the original natural materials. The influence factors on surfactant activity also showed that their surfactivity were enhanced while in acidic conditions, high temperature and binary mixed systems ，but the effects of salinity and the hardness of water on their surfactivity were not obvious. Results showed that while the extracts were compounded in the proportion which calculated and conclued based on Rosen theory, the ability of m(Sapindus Mukorossi Gaertn extracts):m(Gleditsia Sinensis Lam extracts）=1.58:1.00system （systemIII） to form micelles increaed by 76% than the single component while the ability of m(Camellia Oleifera Abel meal extracts) : m(Gleditsia Sinensis Lam extracts)=1.90:1.00 system （systemII）to reduce the surface tension enhanced by 10.5%,which indicated both of them have synergistic effect .Furthermore，the results of foam property testing indicated that ,compared with the single component，the binary mixed systems showed excellent foam property, especially the m(Sapindus Mukorossi Gaertn extracts):m(Gleditsia Sinensis Lam extracts)=1.58:1.00 system（system III） performed best.