The aroma components of tobacco were extracted by supercritical fluid extraction technology from discarded tobacco. In study, we took the extraction yield, the content of carotenoids and major carotenoid degradation products as evaluation indicators.The influence of operation conditions such as entrainer species, the amount of entrainer, extraction temperature, extraction pressure and flow rate of CO2 were investigated. And the essential oils extracted with different entrainers were added into cigarettes as flavors, and sensory evaluation was conducted. The results showed that the most effective entrainer was 1, 2-propanediol. The essential oils extracted with it had better quality, and it effectively improved the taste, enhanced aroma quality, aroma quantity and reduced the irritancy of cigarette smoke. The optimum conditions were choosing 1,2-propanediol as entrainer with 10% by weight, extraction temperature of 40 ℃, extraction pressure of 25 MPa, CO2 flow rate of 2.0 L/min, extract time of 3 h.Under these conditions, the extraction yield was 18.74% and the extraction yield of the content of major carotenoid degradation products was 5.56%.