Abstract: Quaternized curdlan with antibacterial properties was prepared using curdlan as raw material. The chemical structure of quaternized curdlan was confirmed by total reflectance infrared spectroscopy (FTIR-ATR) and 1HNMR spectroscopy. Quaternized curdlan antimicrobial film were prepared by the method of repeated freezing-thawing emulsifying crosslinked. The structure and properties were characterized by scanning electron microscopy (SEM), FTIR-ATR and thermogravimetry (TG). Casting solution stability and water vapor permeability of the films under different formulations were studied. Results show that after 7 days, the casting solution that the mass fraction of paraffin was between 0.4%~0.8% is still opaque milky white liquid and unstratified, therefore, the casting solution has certain stability. The water vapor transmittance of film was 2.12×10-7(m•h•Pa)-1 when the number of freezing thawing times was 3, the mass fraction of glycerol was 2% and the mass fraction of paraffin was 0.6%. The antimicrobial test results show that the quaternized curdlan film had antibacterial effect against Escherichia coli, Staphylococcus and Fusarium but the antibacterial activities of quaternized curdlan film against Staphylococcus and Fusarium were much better than Escherichia coli.