Polyhexamethylene adipate glycol (PHA), polybutylenes adipate glycol (PBA), isophorone diisocyanate (IPDI) and 2,2-dimethylolbutanoic (DMBA) as monomers, trimethylol propane (TMP) as crosslinking agent, and ethylenediamine as chain extender, a series of crosslinked waterborne polyurethane self-matting resin emulsion were prepared and then prepared them into films . The characteristic groups of emulsion were analyzed by FTIR. The morphology of surface and particles were investigated by SEM and TEM. And the glossiness, water resistance, acid and alkali resistance, and the thermodynamic properties of tatex film were also detected. The results showed that the film surface was rough, the average particle size was above 1000nm and the degree of gloss was about 2.0,these satisfy extinction requirement. When there was no crosslinking agent added, the tensile strength was 8.3MPa, Tg was -45℃and the temperature of the film decomposited 10% was 275.7℃. And water absorption rate in deionized water,acid or base solution were 29.1%、27% and 17.2%, respectively. When the mass fraction of crosslinker was 0.65%, the tensile strength increased to 21.4MPa, Tg increased to -40℃ and the temperature of the film decomposited 10% increased to 305.6℃. And water absorption rate in deionized water,acid or base solution were 3.2%、6.2% and 5%, respectively.