Polymethyl acrylate (PMA) was synthesized using methyl acrylate (MA) as raw materials, and polyacryloyl hydrazide (PAH) was prepared by means of the aminolysis of PMA with hydrazine hydrate. Hyaluronic acid (HA) was partially oxidized by sodium periodate to obtain oxidized hyaluronic acid (oxi-HA), and a series of oxidized hyaluronic acid/polyacryloyl hydrazide (oxi-HA/PAH) hydrogels were prepared via Schiff base reaction of oxi-HA and PAH. The structures of PMA, PAH, and oxi-HA were characterized utilizing 1H NMR and FTIR, the molecular weight of PMA was determined using multi-angle laser light scattering system (MALLS). The gelling time, microtopography, swelling ratio, degradation behavior, rheological properties and in vitro drug release performance of hydrogels were investigated via the vial inversion method, SEM, weighing method, rhelogy, and Bradford method, respectively. The results showed that the gelling time of the series of oxi-HA/PAH hydrogels ranged from 8 s to 205 s, and the hydrogels with continuous three-dimensional network structure exhibited suitable viscoelasticity and mechanical strength. In phosphate buffer solution (PBS, pH 7.4) at 37 ℃, the swelling ratio of hydrogels ranged from 12 to 22 after 7 hours, the degradation percentage of oxi-HA60/PAH30 hydrogel was about 66% after 20 days, and the cumulative release percentage of bovine serum albumin (BSA) as model protein drug from drug-loaded oxi-HA60/PAH30 hydrogel was about 86% after 10 days.