In this paper, we took fractional steps to synthesize linear multidentate Schiff base amine ligand [N,N-bis(2-dihydroxyphenyl-amidoethyl)-2,6-pyridinedicarboxylic diamide (L)] firstly, then the 5 new transition metals complexes linked by Shiff base amine. The ligand and complexes are characterized by elemental analysis, IR, UV-VIS and IH NMR. And also the rational structures of them are speculated.The inhibitory activities of compounds on Escherichia coli were studied by micro- dilution. The results appear that Co and Zn complexes show good inhibitory effect on Escherichia coli.