In order to improve the solubility and bioavailability of β-carotene, we studied that the β-carotene was emulsified and encapsulated by Pickering emulsions stabilized with starch nanocrystals (SNC) as a granular emulsifier. The results showed that when the concentration of SNC was 1 wt.%, the volume fraction of oil was 0.5, the concentration of β-carotene was 0.05 wt.%, and the Pickering emulsion was modified by quaternized chitosan (QCS), the emulsion separation phenomenon of the emulsion carrying β-carotene can be improved obviously, and the storage stability is better. At the same time, the in vitro simulated digestion experiment showed that the digestion degree of the oil in the emulsion after QCS modification was increased from 56.68 ? 1.56% to 71.70 ? 2.13%, and the bioaccessibility of β-carotene was also increased from 33.59 ? 0.82% to 57.62 ? 1.58%. Therefore, QCS modification can not only improve the stability of β-carotene Pickering emulsion, but also improve the digestion degree of oil in the emulsion and the bioaccessibility of β-carotene.