Fluorescent waterborne polyurethane(FWPU1~3) with different molecular weight polyethylene glycol was prepared by in situ polymerization of N,N-dihydroxyethylaniline-β-terpyridine (TPPDA) as fluorescent molecules; different amounts of acrylamide (AM) were introduced into FWPU to prepare a series of polyurethane-polyacrylamide complex fluorescent hydrogels (FWPUH1~6). The structure and fluorescence properties of TPPDA and FWPUH were tested by FTIR, solid UV absorption and fluorescence spectroscopy. The results show that the main UV absorption peak of FWPUH is red shift compared with TPPDA, the maximum fluorescence emission wavelength was blue-shifted, the fluorescence quantum yield increases, and its fluorescence emission intensity increases obviously; with the increase of PEG molecular weight and the increase of AM content, the fluorescence intensity of FWPUH increased. When the soft segment was PEG4000, mass ratio of FWPU and AM were 5:4(FWPUH6), the fluorescence intensity was the largest, and compared with TPPDA, the fluorescence intensity increased by 9.5 times, fluorescence quantum yield also increased by 56%. FWPUH3 reached the maximum equilibrium swelling ratio of 9.95; FWPUH2 had the highest compressive strength (0.70 MPa); the soft glass transition temperature and hard glass transition temperature of FWPUH2 and FWPUH5 were -32.1, -46.0 ℃ and 96.5 and 105.7 ℃.