A Gemini cationic surfactant with diamide groups (ADQ-12) was synthesized using Lauric acid, N, N-dimethyl-1, 3-propanediamine and 1, 3-dichloro-2-propanol as raw materials under the action of sodium carbonate. Product was characterized by FTIR and 1HNMR, the surface/ interfacial activities and application performance were researched. The results showed that ADQ-12 has critical micelle concentration(CMC value is 1.41×10-4 mol/L) that are 1~2 orders of magnitude lower than conventional single cationic surfactants and γCMC is 32.26 mN/m; When the surfactant concentration is 0.05 g/L, the interfacial tension of kerosene/water(IFT) could be reduced to ultra-low level (4.1×10-3 mN/m), which is much lower than 2 mN/m of hexadecyl trimethyl ammonium bromide(CTAB); ADQ-12 showed strong resistance to calcium and magnesium ions(>60 g/L) in the case of concentration of 1.0 g/L; The antibacterial rate of ADQ-12 against on S. aureus and E. coli was still 100% when surfactant concentration is 10 mg/L, which is greater efficacy than didecyl dimethyl ammonium chloride(DDAC); The biodegradation of ADQ-12 after 7 days was higher than 99% and showed the excellent biodegradability.