In this paper, a series of two-block and thri-block polyether reverse demulsifiers were synthesized under 120 ℃ conditions by using trimethyl-hydroxypropyl propane diamine as initiator, potassium hydroxide as catalyst and through open-loop polymerization of ethylene oxide (EO) and propylene oxide (PO) as monomers. The infrared spectra and NMR spectra show that the synthesized products are consistent with the design structure.The demulsification results showed that the reverse demulsifier with tri-block structure and block mass ratio n(EO): n(PO): n(EO)=1:6:12 has a well demulsification performance for slickwater flowback fluid. Its dehydration rate is greater than 97%. The results of dynamic interfacial tension and interfacial dilatation modulus experiments presented that EPO-8 has faster diffusion rate to interface and better performance for decreasing the film strength. That is why it had the best demulsification performance.