Phosphorylated polysaccharides from Tussilago farfara was synthesized . The synthetic process conditions was optimized via the response surface method. The research results show that 6.03h, 86℃, 14.25% of urea，2.0 of mass ratio of mix-phosphate, 1.5 of mass ratio of mix-phosphate and polysaccharides were the optimum conditions. The Ds of phosphorylated polysaccharides from Tussilago farfara was 0.697. The antioxidant ability of phosphorylated polysaccharides from Tussilago farfara had been improved.