The fermentation medium and conditions of artemisinic acid produced by engineered S. cerevisiae 1211, were optimized in this paper. The yield of artemisinic acid was used as evaluation index to investigate the effects of temperature, pH, carbon source, nitrogen source and galactose concentration on the fermentation process by using single factor experiments and response surface methodology.The results showed that the production of artemisinic acid could reach 1529.7±12.6 mg/L under the conditions of temperature 30 °C, pH 5.5, sucrose 91.8 g/L, galactose 10.1 g/L, ammonium sulfate 10.3 g/L and monopotassium phosphate 8.7 g/L, which was increased by 67.1% compared with that in the initial medium. This study provided some reference for the further development of industrial production.