Taking deep eutectic solvent-water mixture (LAEG40) as extraction solvent, extraction yield of salidroside as main index, and extraction yield of tyrosol as auxiliary index, the effects of three main parameters solvent to solid ratio, extraction temperature and extraction time on the simultaneous extraction of salidroside and tyrosol from Rhodiola rosea L were optimized by response surface methodology. The optimal extraction conditions were obtained as follows: solvent to solid ratio of 12.05:1 (mL/g), extraction temperature of 60 ?C and extraction time of 35 min. Under these conditions, the extraction yield of salidroside reached 19.3552?0.6604 mg/g, and the extraction yield of tyrosol reached 1.7211?0.0585 mg/g, which were much higher than those of traditional solvents (water and ethanol). In order to recover the salidroside and tyrosol from the LAEG40 extraction solution, the adsorption and separation method with macroporous resin was carried out. After systematical optimization, the optimal conditions for adsorption and separation were obtained as follows: SP-825 as the adsorption resin, 30 mL LAEG40 extraction solution to 10 g SP-825, 80 % ethanol as the elution solvent, eluting volume of 40 mL, and volume flow at 1mL/min. Under these conditions, the recovery rates of salidroside and tyrosol attained to 60.47 % and 85.07 %, respectively.